Marloth Nature Reserve


Research

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Flower color polymorphisms and ecotypic variation
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Protea repens color morphs

Pink (anthocyanin-rich) and white (anthocyanin-deficient) color morphs of Protea repens

I. Evolutionary maintenance of Protea color polymorphisms from the population to genus-wide levels

This goal of this research is to identify key ecological and evolutionary conditions that promote color polymorphism and monomorphism at broad phylogenetic and landscape scales. My current focus is on the genus Protea, within which 40% of species have co-occurring pink-flowered and white-flowered plants. By comparing traits of pink and white morphs in 10 populations of four species, I demonstrated that white-flowered plants generally produced heavier seeds, more germinable seeds, more flowers per inflorescence, and more nectar (and recieved longer pollinator visits), yet pink-flowered plants were occasionally less susceptible to seed-eating larvae. Increased seed predation thereby offsets the benefits of producing more flowers and higher quality seed for white morphs in some populations, and together these factors promote the maintenance of both morphs in a world where seed predator pressures likely vary over space and time (Carlson and Holsinger 2010, Carlson and Holsinger 2012).

I am currently testing whether these local processes can be 'scaled up' to explain the presence and absence of different color morphs accross the genus Protea. As part of this effort, I have initiated a citizen science project to learn more about where polymorphic versus monomorphic Protea populations occur. Click the link to learn about the Protea flower color survey on iSpot Southern Africa

II. Patterns of ecotypic variation and polymorphism in Hibiscus  species of the Southeastern and Central United States

As a new faculty member at Nicholls State University in Thibodaux, LA, I am now exploring related questions in native Hibiscus species in the United States.  Using the five species of Hibiscus section Muenchhusia, I am beginning to document patterns of phenotypic variation among and within species.  This work will not only address basic questions related to flower color polymorphism, but it will also address applied questions related to local adaptation and resilience to environmental change.  

On the applied side, I seek to determine the extent to which genetically-based variation in traits and plasticity will influence Hibiscus species vulnerability to anthropogenic change.  Using common gardens, I will quantify trait variation and plasticity within H. laevis and H. moscheutos sampled across their distributions.  The goal is to identify differentially-resistant ecotypes and refine assessments of species vulnerability to include physiological and evolutionary adaptation.  My focal questions are: How do functional traits vary within two wide-spread Hibiscus species, and are among-population differences predominately due to plasticity or genetic variation?  How are trait variation and capacity for plasticity related to environmental variation (e.g., climate, disturbance history, and soils)?

My color polymorphism work on Hibiscus will parallel my ongoing polymorphism work in Protea.  As I collect data on Hibiscus trait correlations and phenotypic variation as described above, I will also quantify pigmentation in flowers, leaves, and stems. These data will be directed towards the question of why color polymorphism persists in some species and populations of  Hibiscus section Muenchhusia (e.g., H. moscheutos, H. laevis) but is absent or very rare in the wild for other species  (H. coccineus, H. aculeatus, H. grandiflorus).   

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Hibiscus_laevis_bayou_corne
White and pink color morphs of Hibiscus laevis in Southern Louisiana


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Functional trait evolution in South Africa
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measuring physiology
Measuring photosynthetic rates and stem water potential on Protea obtusifolia

I. Geographic and phylogenetic patterns in drought-related traits of South African Protea and Pelargonium  

Since 2010, I have been involved in a large-scale collaborative project at the University of Connecticut, funded by the NSF DIMENSIONS of Biodiversity program.  The goal of this research is to better understand the evolutionary history of drought-related traits in the genera Protea and Pelargonium in South Africa, which should help refine predictions about their likely responses to climate change.  My focus, beginning as a postdoctoral researcher, has been to establish a 700-plant common garden of Protea repens and to direct  field sampling of Protea species in South Africa.  Thus far, we have collected genetic material and functional trait data on 55 species from 87 populations. Follow this link for the UCONN Dimensions of Biodiversity Wiki page.

II. Adaptive differentation and plasticity in Protea sect. Exsertae 

From 2007-2010, I was  involved in another NSF-funded project investigating the mode of evolution within the white proteas, a monophyletic clade endemic to the Cape region of South Africa. Species in this clade include Protea aurea, P. lacticolor, P. punctata, P. mundii, P. venusta, and P. subvestita. Our research asked if these species diversified predominately through adaptive (habitat specialization) or non-adaptive (geographic isolation and drift) processes. This work was in collaboration with the South African National Biodiversity Institute and Rachel Prunier, and the principal investigator was Kent Holsinger. 

Our research methods combined field-based ecological measurements, common garden experiments, molecular data, and phylogenetic analyses. I was based part-time in Cape Town, collecting seeds and data in wild populations from 35 locations across the Western Cape, Eastern Cape, and KwaZulu-Natal and maintaining two common gardens. My focus was on determining if among-population differences in vegetative traits, physiology and extent of plasticity are related to natural selection and local adaptation within the clade. By comparing selection gradients in the wild and between the common gardens, we have evidence that ecologically-based divergent selection maintains some of the differences in SLA, leaf area and growth among white protea populations (Carlson et al. 2011; see also Prunier et al. 2012; Carlson and Holsinger in press)


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Natural and sexual selection on floral traits
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Chrysothemis and Phaethornis
TopChrysothemis friedrichsthaliana bud and open flower. Middle: Adult Alucita moth, which oviposits in unopened buds and whose larvae then consume developing floral parts. Bottom: Little hermit (Phaethornis striigularis subsp. striigularis) and Chrysothemis friedrichsthaliana male and female phase flowers

I. Pollinator-mediated sexual selection on nectar production

My dissertation research focused on the interactions between a Neotropical plant species and its floral enemies and pollinators. Chrysothemis friedrichsthaliana [Gesneriaceae] is a hummingbird-pollinated herb found in lowland rainforests throughout Central America. In one series of experiments, I linked floral rewards, pollinator behavior and plant reproductive success. My objective was to test adaptive hypotheses for male-biased nectar production, i.e., increased nectar production during the male relative to female phase of dichogamous flowers (reviewed in Carlson and Harms 2006). Male-biased nectar production, as is seen in this and > 15 other species, results in variable floral rewards between flowers on a plant, as well as an increased incentive for pollinators to visit male-phase flowers. I demonstrated that in Chrysothemis, male-biased nectar production was partially explained by pollinator-mediated selection on traits that more strongly promote pollen removal as opposed to pollen delivery (i.e., sexual selection). I found limited evidence that selection to reduce geitonogamy could also contribute to the maintenance of the trait (Carlson 2007, 2008). 

II. Liquid defense against floral herbivory 

I also examined floral herbivory and the functional significance of the water calyx of C. friedrichsthaliana. The enlarged, cup-like calyx holds and secretes liquid, such that immature flowers develop under water. In a calyx draining experiment, I demonstrated that a highly detrimental microlepidopteran herbivore (Alucitidae), was partially deterred by the water-filled calyces, relative to calyces without water. These results suggest that the water calyx of Chrysothemis functions in part as a physical floral defense (Carlson and Harms 2007).